Journal: Biotechnology for Biofuels and Bioproducts

Article Title: Metabolic engineering to improve production of 3-hydroxypropionic acid from corn-stover hydrolysate in Aspergillus species

doi: 10.1186/s13068-023-02288-1

Figure Lengend Snippet: Nutritional requirements for production of 3-HP from sugar monomers

Article Snippet: A. pseudoterreus (ATCC 32359) and A. niger (ATCC 11414) from the American Type Culture Collection (Rockville, MD, USA) were grown on complete medium (CM) or potato dextrose agar (PDA) plates at 30 °C for culture maintenance and spore preparation.

Techniques: Control

Journal: Biotechnology for Biofuels and Bioproducts

Article Title: Metabolic engineering to improve production of 3-hydroxypropionic acid from corn-stover hydrolysate in Aspergillus species

doi: 10.1186/s13068-023-02288-1

Figure Lengend Snippet: Parent and transgenic strains of A. pseudoterreus and A. niger used in this study

Article Snippet: A. pseudoterreus (ATCC 32359) and A. niger (ATCC 11414) from the American Type Culture Collection (Rockville, MD, USA) were grown on complete medium (CM) or potato dextrose agar (PDA) plates at 30 °C for culture maintenance and spore preparation.

Techniques: Transgenic Assay

Journal: bioRxiv

Article Title: Comparative evaluation of Aspergillus niger strains for endogenous pectin depolymerization capacity and suitability for D-galacturonic acid production

doi: 10.1101/858548

Figure Lengend Snippet: Schematic overview listing selected but representative members of openly available and related A. niger strains based on phylogeny . Depicted are commonly used lab strains (green, right) vs. the industrially adapted strains (blue, left) including the citric acid producer clade (light blue) and the more distantly related enzyme producers (dark blue). Yellow signs indicate instances of UV-mutagenesis, grey gear wheels symbolize targeted genomic engineering steps and red boxes indicate strains used in this study.

Article Snippet: We therefore systematically compared the pectinolytic capabilities of six A. niger strains (ATCC 1015, ATCC 11414, NRRL 3122, CBS 513.88, NRRL 3, N402) using controlled batch cultivations in stirred-tank bioreactors.

Techniques: Mutagenesis

Journal: bioRxiv

Article Title: Comparative evaluation of Aspergillus niger strains for endogenous pectin depolymerization capacity and suitability for D-galacturonic acid production

doi: 10.1101/858548

Figure Lengend Snippet: (A – D) BDW of A. niger NRRL 3 (A, •), A. niger N402 (B, ♦), A. niger ATCC 1015 (C, ■) and A. niger ATCC 11414 (D, ▲) during 90 h submerged batch cultivations in a 3 L stirred-tank bioreactor with 2% pectin minimal medium. (E) Morphological changes of all four A. niger strains throughout the cultivations are shown below. White scale bars indicate 1 mm. Lines in A – D indicate morphology sampling times.

Article Snippet: We therefore systematically compared the pectinolytic capabilities of six A. niger strains (ATCC 1015, ATCC 11414, NRRL 3122, CBS 513.88, NRRL 3, N402) using controlled batch cultivations in stirred-tank bioreactors.

Techniques: Sampling

Journal: bioRxiv

Article Title: Comparative evaluation of Aspergillus niger strains for endogenous pectin depolymerization capacity and suitability for D-galacturonic acid production

doi: 10.1101/858548

Figure Lengend Snippet: (A – D) Total secreted protein concentrations (grey) in culture su-pernatants of pre-selected A. niger strains in 2% pectin minimal medium over a time course of 90 h. (E – H) Total (black) and endo-PGase activities (white) of culture supernatants throughout cultivation.

Article Snippet: We therefore systematically compared the pectinolytic capabilities of six A. niger strains (ATCC 1015, ATCC 11414, NRRL 3122, CBS 513.88, NRRL 3, N402) using controlled batch cultivations in stirred-tank bioreactors.

Techniques:

Journal: bioRxiv

Article Title: Comparative evaluation of Aspergillus niger strains for endogenous pectin depolymerization capacity and suitability for D-galacturonic acid production

doi: 10.1101/858548

Figure Lengend Snippet: Black and white triangles (Δ, ▲) represent replicates of hydrolysis using 96 h culture supernatants of A. niger ATCC 11414 stirred tank batch cultivations in 2% pectin minimal medium, with respective dashed lines in grey and black. Black and white diamonds (♦, ◊) represent two replicates using supernatants of N402 with respective dotted lines in black and grey.

Article Snippet: We therefore systematically compared the pectinolytic capabilities of six A. niger strains (ATCC 1015, ATCC 11414, NRRL 3122, CBS 513.88, NRRL 3, N402) using controlled batch cultivations in stirred-tank bioreactors.

Techniques:

Journal: BMJ Open Diabetes Research & Care

Article Title: Plasma C1q/tumor necrosis factor-related protein-3 concentrations are associated with diabetic peripheral neuropathy

doi: 10.1136/bmjdrc-2021-002746

Figure Lengend Snippet: Plasma C1q/tumor necrosis factor-related protein-3 (CTRP3) concentrations in patients without diabetic peripheral neuropathy (non-DPN) and with DPN. Data are presented as means±SD; ***p<0.001 compared with non-DPN.

Article Snippet: The plasma CTRP3 concentrations were determined by ELISA with specific antibody against CTRP3 (Proteintech, Wuhan, China) according to standard protocols.

Techniques: Clinical Proteomics

Journal: BMJ Open Diabetes Research & Care

Article Title: Plasma C1q/tumor necrosis factor-related protein-3 concentrations are associated with diabetic peripheral neuropathy

doi: 10.1136/bmjdrc-2021-002746

Figure Lengend Snippet: The results of the nerve conduction testing from groups divided according to plasma CTRP3 tertiles

Article Snippet: The plasma CTRP3 concentrations were determined by ELISA with specific antibody against CTRP3 (Proteintech, Wuhan, China) according to standard protocols.

Techniques: Clinical Proteomics

Journal: BMJ Open Diabetes Research & Care

Article Title: Plasma C1q/tumor necrosis factor-related protein-3 concentrations are associated with diabetic peripheral neuropathy

doi: 10.1136/bmjdrc-2021-002746

Figure Lengend Snippet: Scatter plots showing the correlations of plasma C1q/tumor necrosis factor-related protein-3 (CTRP3) concentrations with nerve conduction parameters. The correlations were assessed by Pearson’s correlation test. M represents motor, S represents sensory, L represents left, R represents right, T represents tibia, P represents peroneal and Su represents sural.

Article Snippet: The plasma CTRP3 concentrations were determined by ELISA with specific antibody against CTRP3 (Proteintech, Wuhan, China) according to standard protocols.

Techniques: Clinical Proteomics